https://doi.org/10.5125/jkaoms.2011.37.5.415
J Korean Assoc Oral Maxillofac Surg 2011; 37(5): 415~420
Interaction between odontoblast and bio-calcium phosphate cement reinforced with chitosan
Byung-Do Chun1, Sung-Won Kim1, Sung-Tak Lee1, Tae-Hoon Kim1, Jung-Han Lee1, Gyoo-Cheon Kim2, Yong-Deok Kim1, Uk-Kyu Kim1
© Korean Association of Oral and Maxillofacial Surgeons. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Purpose: Calcium phosphate cement (CPC) is one of many useful materials for restoring tooth defects, periodontium and maxillofacial area.
Chitosan is a biodegradable material that has been shown to promote the growth and differentiation of osteoblasts in culture. This study examined the
interaction between odontoblasts and bio-calcium phosphate cement reinforced with chitosan.
Materials and Methods: 5×103 odontoblastic cells were seeded into each well. Various concentrations of bio-calcium phosphate cement reinforced
with chitosan (10, 20, 50, 100, 200, 500 μg/ml, 1, 2, 4 mg/ml) were diluted and added to the wells. The well was incubated for 24 h, 48 h and
72 h. After incubation, the number of cells was assessed to determine the cell viability. A cytokinesis-block micronucleus assay and chromosomal
aberration test were carried out to estimate the extent of chromosomal abnormalities. Microscopic photographs and RT-PCR were performed to examine
the adhesion potential of bio-calcium phosphate cement reinforced with chitosan.
Results: Bio-CPC-reinforced chitosan did not show significant cytotoxicity. The number of damaged chromosomes in the cells treated with Bio-
CPC-reinforced chitosan was similar to that in the control cells. There was no significant increase in the number of chromosomal aberrations in the
Bio-CPC reinforced chitosan exposed cells. Microscopic photographs and RT-PCR confirmed the adhesive potential of bio-CPC reinforced chitosan
to odontoblasts.
Conclusion: Bio-CPC-reinforced chitosan did not affect the odontoblastic cell viability, and had no significant cytotoxic effect. Bio-CPC-reinforced
chitosan showed adhesive potential to odontoblasts. These results are expected form the basis of future studies on the effectiveness of dental restorative
materials in Bio-CPC reinforced with chitosan.
Keywords: Calcium phosphate cement, Chitosan, Odontoblast

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