J Korean Assoc Oral Maxillofac Surg 2009; 35(4): 199~204
Xenografted Tumorigenesis in the oral vestibule of nude mice by
Snail transfection: Histological and immunohistochemical study
Moon-Key Kim, Eun-Ha Lee, Jin Kim, Jong-In Yook, In-Ho Cha
© Korean Association of Oral and Maxillofacial Surgeons. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Purpose: The purpose of this study is to investigate the epithelial-mesenchymal transition (EMT) induced by Snail transcription factor and Snailtransfected
in vivo tumors with histopathological features.
Materials and methods: We induced in vivo xenografted tumorigenesis in the oral vestibules of nude mice by a Snail transfected HaCaT cell line
and investigated morphological and immunohistochemical features in Snail expressive tumors.
Results: We identified tumor masses in 14 out of 15 nude mice in the HaCaT-Snail cell inoculation group, but no tumors were present in any of the
HaCaT cell inoculation group. Induced tumors showed features of poorly differentiated carcinoma with invasion to neighboring muscles and bones.
The HaCaT-Snail tumors showed decreased expressions of E-cadherin and cytokeratin, but showed increased expressions of vimentin and N-cadherin.
Discussion: The Snail transfected xenograft can improve productivity of malignant tumors, show various histopathological features including invasive
growth, and aid in the investigation of tumor progression and the interaction with surrounding tissues.
Keywords: : E-cadherin, neoplastic cell transformation, Snail transcription factor, xenograft model

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