Sequences of primers using polymerase chain reaction reaction
Sense (5’ to 3’) | Antisense (5’ to 3’) | |
---|---|---|
Exon1 | cctgcaaactggtaggcg | cgtgacgtaaagccgaacc |
Exon2 | cccggcctgttgttttctt | aaggccctcctcttttattttt |
Exon3 | caggcatggggtctcactg | tgaaagcaagtatggtttgcag |
Exon4 | agctagctaacttctcaaatcttct | aacctagactgcttccaaggg |
Exon5 | acaggcttccaaatcccag | cctttagaacacaagcccacc |
Exon6 | ttgctgagggccagatgat | tgagctttattaacacatgacaaaa |
Exon7 | aatccccataatttagctctcca | tggcaaatgtgcctctctaca |
Exon8 | tttttgtcaatcatttaaccatc | ctggccaggttccagttct |
Exon9 | ttcaaaagatacactccccaaaa | tttaggaatgcagcaactgaca |
Primers were designed with primer 3 using sequences from GenBank accession number of NG_012329.2.