Journal of the Korean Association of Oral and Maxillofacial Surgeons : eISSN 2234-5930 / pISSN 2234-7550

Table. 1.

Table. 1.

Primer sequences, reaction conditions, and product size for RT-PCR

Gene Sequence Product size (bp) Annealing (℃) Cycles
GAPDH F: ACC ACA GTC CAT GCC ATC AC
R: TTC ACC ACC CTG TTG CTG TA
450 55 25
Collagen1 F: GAA AAC ATC CCA GCC AAG AA
R: CAG GTT GCC AGT CTC CTC AT
270 57 23
Collagen3 F: CAG GTG AAC GTG GAG CTG C
R: TGC CAC ACG TGT TTC CGT GG
661
849
57 23
Osteonectin F: CCA GAA CCA CCA CTG CAA AC
R: GGC AGG AAG AGT CGA AGG TC
161 57 23
Osteopontin F: TCG CAG ACC TGA CAT CCA GT
R: TCG GAA TGC TCA TTG CTC TC
267 57 32
BMP-2 F: GTC CAG CTG TAA GAG ACA CC
R: GTA CTA GCG ACA CCC ACA AC
316 54 31

(RT-PCR: reverse transcription-polymerase chain reaction, GAPDH: glyceraldehyde 3-phosphate dehydrogenase, BMP-2: bone morphogenetic protein-2)

J Korean Assoc Oral Maxillofac Surg 2021;47:454~464
https://doi.org/10.5125/jkaoms.2021.47.6.454
© JKAOMS