J Korean Assoc Oral Maxillofac Surg 2019; 45(2): 91~96
An experimental investigation of the effects of chronic stress on bone-to-implant contact
Serkan Dundar1, Alihan Bozoglan1, Ferhan Yaman2, Mustafa Kirtay3, Ozgur Bulmus4, Hacer Sahin Aydinyurt5, Murat Yavuz Solmaz6, Cenk Yanen6
1Department of Periodontology, Faculty of Dentistry, Firat University, Elazig, 2Private Practice, Oral and Maxillofacial Surgeon, Diyarbakir, Turkey, 3Private Practice, Oral and Maxillofacial Surgeon, Toronto, Canada, 4Department of Physiology, Faculty of Medicine, Firat University, Elazig, 5Department of Periodontology, Faculty of Dentistry, Y?z?nc? Yıl University, Van, 6Department of Mechanical Engineering, Faculty of Engineering, Firat University, Elazig, Turkey
Serkan Dundar
Department of Periodontology, Faculty of Dentistry, Firat University, Campus, Elazig 23119, Turkey, TEL: +90-424-237-00-00/1746 FAX: +90-424-212-27-17, E-mail: dtserkandundar@gmail.com
ORCID: https://orcid.org/0000-0003-3944-1957
Received May 17, 2018; Revised June 29, 2018; Accepted July 2, 2018.; Published online April 30, 2019.
© The Korean Association of Oral and Maxillofacial Surgeons.. All rights reserved.

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Objectives: This study aimed to investigate the effects of chronic restraint stress on the osseointegration of titanium implants.
Materials and Methods: Twenty adult male Wistar albino rats were used in the study. After surgical insertion of titanium implants into the metaphyseal part of the tibial bone, rats were randomly divided into two groups: a control group (CNT group) and an experimental restraint stress group (RS group). In the CNT group, titanium implants were inserted surgically, and rats received no further treatment during the 47-day experimental period. In the RS group, restraint stress was applied for 3 hours per day for 45 days, beginning 2 days after implant surgery. Weight of the rats was measured prior to surgery and at the end of the study to analyze the effects of stress. At the end of the experimental period, rats were euthanized, and implants and surrounding bone tissues were used for undecalcified histological analysis. Serum cortisol levels were assessed in cardiac blood samples from the rats following centrifugation.
Results: Average weight of rats in the RS group was lower than that of rats in the CNT group after the experimental protocol had been completed (P<0.05). Further, serum cortisol levels were higher in the RS group than in the CNT group (P<0.05). There were no significant differences in bone-implant connection levels between the two groups (P>0.05).
Conclusion: The data analyzed in this study suggest that chronic restraint stress did not adversely affect rats during a 45-day osseointegration period.
Keywords: Restraint stress, Osseointegration, Bone-to-implant contact, Rats, Tibia
Fig. 1. Non-decalcified histologic images of the groups (toluidine blue staining,×2). (A) Control group. (B) Restraint stress group.
Serkan Dundar et al: An experimental investigation of the effects of chronic stress on bone-to-implant contact.

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30 April 2020
Vol. 46
No. 2 pp. 85~159

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