J Korean Assoc Oral Maxillofac Surg 2018; 44(6): 259~268
Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts
Seok-Young Jeong1, Ji-Un Hong1, Jae Min Song1, In Ryoung Kim2, Bong Soo Park2, Chul Hoon Kim3, Sang Hun Shin1
Departments of 1Oral and Maxillofacial Surgery and 2Oral Anatomy, School of Dentistry, Pusan National University, Yangsan,
3Department of Oral and Maxillofacial Surgery, Dong-A University Hospital, Busan, Korea
Sang-Hun Shin
Department of Oral and Maxillofacial Surgery, School of Dentistry, Pusan National University, 49 Busandaehak-ro, Mulgeum-eup, Yangsan 50612, Korea
TEL: +82-55-360-5100 FAX: +82-55-360-5104
E-mail: ssh8080@pusan.ac.kr
ORCID: https://orcid.org/0000-0002-0467-6206
Received March 14, 2018; Revised June 3, 2018; Accepted June 8, 2018.; Published online December 31, 2018.
© Korean Association of Oral and Maxillofacial Surgeons. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Objectives: The purpose of this study was to evaluate the synergic effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) and low-level laser therapy (LLLT) on bisphosphonate-treated osteoblasts.
Materials and Methods: Human fetal osteoblast cells (hFOB 1.19) were cultured with 100 µM alendronate. Low-level Ga-Al-As laser alone or with 100 ng/mL rhBMP-2 was then applied. Cell viability was measured with MTT assay. The expression levels of receptor activator of nuclear factor kappa-B ligand (RANKL), macrophage colony-stimulating factor (M-CSF), and osteoprotegerin (OPG) were analyzed for osteoblastic activity inducing osteoclastic activity. Collagen type and transforming growth factor beta-1 were also evaluated for bone matrix formation.
Results: The results showed that rhBMP-2 and LLLT had a synergic effect on alendronate-treated osteoblasts for enhancing osteoblastic activity and bone matrix formation. Between rhBMP-2 and LLLT, rhBMP-2 exhibited a greater effect, but did not show a significant difference.
Conclusion: rhBMP-2 and LLLT have synergic effects on bisphosphonate-treated osteoblasts through enhancement of osteoblastic activity and bone formation activity.
Keywords: Alendronate, Osteoblasts, Bone morphogenetic protein 2, Low-level laser therapy


Current Issue

31 December 2018
Vol. 44
No. 6 pp. 249~302

Indexed in