J Korean Assoc Oral Maxillofac Surg 2018; 44(3): 120~127
Expression of collagenases (matrix metalloproteinase-1, 8, 13) and tissue inhibitor of metalloproteinase-1 of retrodiscal tissue in temporomandibular joint disorder patients
Won Gyung Gho1, Yuri Choi2, Kwang-Ho Park2, Jong-Ki Huh2
1Department of Dentistry, Myongji Hospital, Goyang,
2Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry, Seoul, Korea
Jong-Ki Huh
Department of Oral and Maxillofacial Surgery, Gangnam Severance Hospital, Yonsei University College of Dentistry, 211 Eonju-ro, Gangnamgu, Seoul 06273, Korea
TEL: +82-2-2019-4560 FAX: +82-2-3463-4052
E-mail: omshuh@yuhs.ac
ORCID: https://orcid.org/0000-0002-7381-3972
Received December 20, 2017; Revised February 9, 2018; Accepted February 12, 2018.; Published online June 30, 2018.
© Korean Association of Oral and Maxillofacial Surgeons. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
 Abstract
Objectives: The aim of this study was to reveal how collagenases (matrix metalloproteinase [MMP]-1, 8, 13) and tissue inhibitor of metalloproteinase 1 (TIMP-1) are expressed in immunohistochemistry of retrodiscal tissue in temporomandibular joint disorder patients.
Materials and Methods: This study was conducted on 39 patients who underwent discoplasty or discectomy. Immunohistochemical staining was undertaken and expression levels of MMP-1, 8, 13, and TIMP-1 were evaluated. The status of internal derangement of disc, osteoarthritis, and joint effusion were analyzed using magnetic resonance imaging (MRI). Disc status observed during operation was also categorized.
Results: The more severe disc derangement was observed on MRI, the more increased expression of MMPs and TIMP-1 appeared. Regarding MMP-13 expression, 86.7% of late-stage disc displacement patients showed grade II or III. Expression level of MMPs or TIMP was not statistically significant associated with joint effusion level. In perforation and/or adhesion groups, all patients showed grade II or III expression of MMP-13. Once perforation occurred, MMP-13 showed increased expression with statistical significance.
Conclusion: MMP-1 and MMP-13 expression seem to be related to progression of osteoarthritis whereas MMP-8 does not seem to have a specific role with regard to temporomandibular joint disorders. TIMP-1 is considered to be partly related to internal derangement rather than osteoarthritis, but it is not significant.
Keywords: Collagenases, Matrix metalloproteinase, Tissue inhibitor of metalloproteinase, Temporomandibular joint disorders, Immunohistochemistry


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31 August 2018
Vol. 44
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