J Korean Assoc Oral Maxillofac Surg 2017; 43(6): 373~387
Stepwise verification of bone regeneration using recombinant human bone morphogenetic protein-2 in rat fibula model
Jung-Woo Nam1,2, Hyung-Jun Kim1,3,4
1Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry, Seoul,
2Department of Oral and Maxillofacial Surgery, Wonkwang University Sanbon Hospital, Gunpo,
3Oral Cancer Research Institute and 4Research Institute for Dental Biomaterials & Bioengineering, Yonsei University College of Dentistry, Seoul, Korea
Hyung-Jun Kim
Department of Oral and Maxillofacial Surgery, Yonsei University College of Dentistry, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Korea
TEL: +82-2-2228-3132 FAX: +82-2-2227-8256
E-mail: kimoms@yuhs.ac
ORCID: http://orcid.org/0000-0002-3364-9995
Received July 22, 2017; Revised September 14, 2017; Accepted September 20, 2017.; Published online December 31, 2017.
© Korean Association of Oral and Maxillofacial Surgeons. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objectives: The purpose of this study was to introduce our three experiments on bone morphogenetic protein (BMP) and its carriers performed using the critical sized segmental defect (CSD) model in rat fibula and to investigate development of animal models and carriers for more effective bone regeneration.
Materials and Methods: For the experiments, 14, 16, and 24 rats with CSDs on both fibulae were used in Experiments 1, 2, and 3, respectively. BMP-2 with absorbable collagen sponge (ACS) (Experiments 1 and 2), autoclaved autogenous bone (AAB) and fibrin glue (FG) (Experiment 3), and xenogenic bone (Experiment 2) were used in the experimental groups. Radiographic and histomorphological evaluations were performed during the follow-up period of each experiment.
Results: Significant new bone formation was commonly observed in all experimental groups using BMP-2 compared to control and xenograft (porcine bone) groups. Although there was some difference based on BMP carrier, regenerated bone volume was typically reduced by remodeling after initially forming excessive bone.
Conclusion: BMP-2 demonstrates excellent ability for bone regeneration because of its osteoinductivity, but efficacy can be significantly different depending on its delivery system. ACS and FG showed relatively good bone regeneration capacity, satisfying the essential conditions of localization and release-control when used as BMP carriers. AAB could not provide release-control as a BMP carrier, but its space-maintenance role was remarkable. Carriers and scaffolds that can provide sufficient support to the BMP/carrier complex are necessary for large bone defects, and AAB is thought to be able to act as an effective scaffold. The CSD model of rat fibula is simple and useful for initial estimate of bone regeneration by agents including BMPs.
Keywords: Bone regeneration, Bone morphogenetic proteins, Carrier, Autoclaved autogenous bone, Rat fibula

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30 April 2018
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