J Korean Assoc Oral Maxillofac Surg 2017; 43(5): 299~304
Rabbit submandibular salivary gland replantation
Akram A. Almansoori1,2, Namuun Khentii1, Wei-Hong Hei3, Nari Seo4, Sung-Ho Lee4, Soung Min Kim1, Jong Ho Lee1,4,5
1Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea,
2Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Sana’a University, Sana’a, Yemen,
3The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China,
4Dental Research Institute, Seoul National University, 5Oral Cancer Center, Seoul National University Dental Hospital, Seoul, Korea
Jong Ho Lee
Department of Oral and Maxillofacial Surgery, Seoul National University Dental Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Korea
TEL: +82-2-2072-2630 FAX: +82-2-766-4948
E-mail: leejongh@snu.ac.kr
ORCID: http://orcid.org/0000-0002-8843-545X
Received April 7, 2017; Revised June 23, 2017; Accepted July 5, 2017.; Published online October 31, 2017.
© Korean Association of Oral and Maxillofacial Surgeons. All rights reserved.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Objectives: To test the feasibility of submandibular salivary gland (SMG) replantation techniques and the survival of the replanted glands. Such a study can provide a rationale for later allotransplantation procedures, along with implementation of conventional and advanced immunosuppression therapy.
Materials and Methods: Six SMG replantations were performed in New Zealand white rabbits. One week postoperatively, 99mTc scintigraphy was performed and the uptake ratio and salivary excretion fraction were calculated. Two to four weeks later, submandibular glands were excised, fixed, and stained with H&E for histomorphometric evaluation.
Results: Intraoperatively, all glands showed patent blood perfusion except gland 5. Positive tracer uptake and saliva excretion were documented by scintigraphy. On excision, all of the glands except glands 4 and 5 looked viable, with a red color and patent pedicles. Gland 4 was infected and filled with creamy pus, while gland 5 looked pale and necrotic. Histologically, glands 1, 2, 3, and 6 had preserved normal glandular tissue with slight variations from the contralateral normal glands, as their parenchyma was composed of mildly atrophic acini.
Conclusion: Four out of six replanted SMGs successfully survived. The glands maintained good viability and function. Such success depends on safe harvesting, short anastomosis time, and strict control of infection.
Keywords: Rabbits, Submandibular gland, Replantation

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31 October 2017
Vol. 43
No. 5 pp. 287~360

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